Plasmido puc19 pdf files

Click add files and select the files you want to include in your pdf. The puc18puc19 sequence is stored as a pdffile on the genaxxon webpage. Use text editor or plasmid mapping software to view sequence. Efficient cleavage requires at least two copies of the bspmi recognition sequence.

To test this hypothesis, we attempted to insert a 1. It carries a 54 bp multiple cloning site polylinker. Dna fragments into puc19 vector to study the ligation. Sticky ends from different bfuai sites may not be compatible. How to merge pdfs and combine pdf files adobe acrobat dc. Im planing to insert a big fragment into li for expresing some genes. Thermo scientific puc19 vector is a small, high copy number, e. The designation puc is derived from the classical p prefix. The pmb1 replicon rep responsible for the replication of plasmid source plasmid pbr322. How stable would puc19 be if i insert a big fragment 67kb on its mcs. To transform plasmid dna, puc19, into a bacterial strain.

Efficient cleavage requires at least two copies of the bfuai recognition sequence. Why are two different restriction enzymes used to cut the. Ligation experiments were conducted to examine the ligation efficiencies of hindiii digested puc19 and. You can merge pdfs or a mix of pdf documents and other files. Use with snapgene software or the free viewer to visualize additional data and align other sequences. Plasmid sequence and snapgene enhanced annotations. Why are two different restriction enzymes used to cut the puc19 plasmid and the lux gene dna. How stable would puc19 be if i insert a big fragment 6. High copy number of the puc plasmid results from a romropsuppressible point mutation in rna ii.

This vector is not available from the plasmid repository and is provided here only as a reference. Plasmid dna from escherichia coli rri has been used in polymerase chain reaction pcr. Pdf high copy number of the puc plasmid result from a. The molecule is doublestranded circle, 2686 base pairs in length, and has a high copy number. The puc19 plasmid 2,686 bp confers ampicillin resistance and complement defects in. Bfuai is typically used at 50c, but is 50% active at 37c. Click, drag, and drop to reorder files or press delete to remove any content you dont want. What would have happened if only the hind iii enzyme was 2850178. It contains identical multiple cloning site mcs as puc18. The effect of increasing plasmid size on transformation. A repository of over 200,000 plasmids including protein structure initiative protein expression plasmids and vectors, over 75,000 human plasmids, and whole genome collections from many organisms. The puc19 backbone of pgfp provides a high copy number origin of replication and ampicillin resistance gene for propagation in e.